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tet plko neo vector  (Addgene inc)


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    Structured Review

    Addgene inc tet plko neo vector
    Tet Plko Neo Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tet+plko+neo+vector/Tet-pLKO-neo+(Plasmid+%2321916)/pm38943644-188-9-11
    Average 94 stars, based on 63 article reviews
    tet plko neo vector - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Cloning:

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts.
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 mg/ml). shRNAresistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA (Baillat et al., 2005) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 μg/ml). shRNA-resistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA ( ) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..

    Sequencing:

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts.
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 mg/ml). shRNAresistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA (Baillat et al., 2005) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 μg/ml). shRNA-resistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA ( ) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..

    Plasmid Preparation:

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts.
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 mg/ml). shRNAresistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA (Baillat et al., 2005) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..

    Article Title: Targeting UBR5 inhibits postsurgical breast cancer lung metastases by inducing apoptosis mediated by CDC73 and p53
    Article Snippet: .. To construct the pLKO-Tet-shCdc73 plasmid, Cdc73-shRNA (mCdc73-shRNA: CTCGTTTGGAAGGCCATAAAG) was inserted into the Tet-pLKO-neo vector (Addgene, plasmid #21916). .. To construct the pLKO-shUBR5 plasmid, UBR5-shRNA (hUBR5-shRNA: GCTCGTCTTGATCTACTTTAT) was cloned into the pLKO.1-puro vector (Addgene plasmid #8453).

    Article Title: Targeting UBR5 inhibits postsurgical breast cancer lung metastases by inducing CDC73 and p53 mediated apoptosis.
    Article Snippet: .. To construct pLKO-Tet-shUbr5, pLKO-tet-scrambled and pLKO-TetshCdc73 plasmids, Ubr5-shRNA (sequences are listed in Table S1) and scrambled shRNA Cdc73-shRNA were cloned into the Tet-pLKO-puro (Addgene, plasmid # 21915) and Tet-pLKO-neo vector (Addgene, plasmid #21916). .. To construct the pLKO-shUBR5 and pLKOshCDC73 plasmid, UBR5-shRNA and CDC73-shRNA was cloned into the pLKO.1-puro (Addgene, plasmid #8453) and pLKO.1-neo vector (Addgene, plasmid #13425).

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 μg/ml). shRNA-resistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA ( ) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..

    Article Title: Translocational attenuation mediated by the PERK-SRP14 axis is a protective mechanism of unfolded protein response.
    Article Snippet: .. The annealed shNC and shHRD1 oligonucleotides were ligated into Tet-pLKO-neo vector (Addgene #21916) by using the AgeI and EcoRI restriction sites. ..

    Article Title: Targeting UBR5 inhibits postsurgical breast cancer lung metastases mediated by CDC73 and p53
    Article Snippet: .. To construct the pLKO-Tet-shCdc73 plasmid, Cdc73-shRNA (mCdc73-shRNA: CTCGTTTGGAAGGCCATAAAG) was inserted into the Tet-pLKO-neo vector (Addgene, plasmid #21916). .. To construct the pLKO-shUBR5 plasmid, UBR5-shRNA (hUBR5-shRNA: GCTCGTCTTGATCTACTTTAT) was cloned into the pLKO.1-puro vector (Addgene plasmid #8453).

    Article Title: NBS1-CtIP–mediated DNA end resection suppresses cGAS binding to micronuclei
    Article Snippet: Panels of human cGAS-specific shRNAs (TRCN0000128706, TRCN0000128310, TRCN0000149984, TRCN0000146282 and TRCN0000150010), human NBS1-specific shRNAs (TRCN0000295898, TRCN0000040137, TRCN0000040133, TRCN0000012671 and TRCN0000012672), human CtIP-specific shRNAs (Sigma, TRCN0000318738, TRCN0000005403, and TRCN0000005405) and human RNF20-specific shRNAs (Sigma, TRCN0000033876, TRCN0000033877 and TRCN0000033878) were purchased from Sigma. .. To make tetracycline-inducible shNBS1 RNA constructs, we annealed shNBS1-F1/F2/F3 and shNBS1-R1/R2/R3 primers ( ) in annealing buffer (100 mM NaCl, 10 mM Tris–HCl, pH 7.4), then ligated them into AgeI-EcoRI sites of a Tet-pLKO-neo vector (was a gift from Charles Rudin; Addgene plasmid #47541). ..

    Clone Assay:

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts.
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 mg/ml). shRNAresistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA (Baillat et al., 2005) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..

    Article Title: Targeting UBR5 inhibits postsurgical breast cancer lung metastases by inducing CDC73 and p53 mediated apoptosis.
    Article Snippet: .. To construct pLKO-Tet-shUbr5, pLKO-tet-scrambled and pLKO-TetshCdc73 plasmids, Ubr5-shRNA (sequences are listed in Table S1) and scrambled shRNA Cdc73-shRNA were cloned into the Tet-pLKO-puro (Addgene, plasmid # 21915) and Tet-pLKO-neo vector (Addgene, plasmid #21916). .. To construct the pLKO-shUBR5 and pLKOshCDC73 plasmid, UBR5-shRNA and CDC73-shRNA was cloned into the pLKO.1-puro (Addgene, plasmid #8453) and pLKO.1-neo vector (Addgene, plasmid #13425).

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 μg/ml). shRNA-resistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA ( ) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..

    Mutagenesis:

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts.
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 mg/ml). shRNAresistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA (Baillat et al., 2005) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 μg/ml). shRNA-resistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA ( ) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..

    Transfection:

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts.
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 mg/ml). shRNAresistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA (Baillat et al., 2005) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 μg/ml). shRNA-resistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA ( ) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..

    Construct:

    Article Title: Targeting UBR5 inhibits postsurgical breast cancer lung metastases by inducing apoptosis mediated by CDC73 and p53
    Article Snippet: .. To construct the pLKO-Tet-shCdc73 plasmid, Cdc73-shRNA (mCdc73-shRNA: CTCGTTTGGAAGGCCATAAAG) was inserted into the Tet-pLKO-neo vector (Addgene, plasmid #21916). .. To construct the pLKO-shUBR5 plasmid, UBR5-shRNA (hUBR5-shRNA: GCTCGTCTTGATCTACTTTAT) was cloned into the pLKO.1-puro vector (Addgene plasmid #8453).

    Article Title: Targeting UBR5 inhibits postsurgical breast cancer lung metastases by inducing CDC73 and p53 mediated apoptosis.
    Article Snippet: .. To construct pLKO-Tet-shUbr5, pLKO-tet-scrambled and pLKO-TetshCdc73 plasmids, Ubr5-shRNA (sequences are listed in Table S1) and scrambled shRNA Cdc73-shRNA were cloned into the Tet-pLKO-puro (Addgene, plasmid # 21915) and Tet-pLKO-neo vector (Addgene, plasmid #21916). .. To construct the pLKO-shUBR5 and pLKOshCDC73 plasmid, UBR5-shRNA and CDC73-shRNA was cloned into the pLKO.1-puro (Addgene, plasmid #8453) and pLKO.1-neo vector (Addgene, plasmid #13425).

    Article Title: Targeting UBR5 inhibits postsurgical breast cancer lung metastases mediated by CDC73 and p53
    Article Snippet: .. To construct the pLKO-Tet-shCdc73 plasmid, Cdc73-shRNA (mCdc73-shRNA: CTCGTTTGGAAGGCCATAAAG) was inserted into the Tet-pLKO-neo vector (Addgene, plasmid #21916). .. To construct the pLKO-shUBR5 plasmid, UBR5-shRNA (hUBR5-shRNA: GCTCGTCTTGATCTACTTTAT) was cloned into the pLKO.1-puro vector (Addgene plasmid #8453).

    Article Title: NBS1-CtIP–mediated DNA end resection suppresses cGAS binding to micronuclei
    Article Snippet: Panels of human cGAS-specific shRNAs (TRCN0000128706, TRCN0000128310, TRCN0000149984, TRCN0000146282 and TRCN0000150010), human NBS1-specific shRNAs (TRCN0000295898, TRCN0000040137, TRCN0000040133, TRCN0000012671 and TRCN0000012672), human CtIP-specific shRNAs (Sigma, TRCN0000318738, TRCN0000005403, and TRCN0000005405) and human RNF20-specific shRNAs (Sigma, TRCN0000033876, TRCN0000033877 and TRCN0000033878) were purchased from Sigma. .. To make tetracycline-inducible shNBS1 RNA constructs, we annealed shNBS1-F1/F2/F3 and shNBS1-R1/R2/R3 primers ( ) in annealing buffer (100 mM NaCl, 10 mM Tris–HCl, pH 7.4), then ligated them into AgeI-EcoRI sites of a Tet-pLKO-neo vector (was a gift from Charles Rudin; Addgene plasmid #47541). ..

    shRNA:

    Article Title: Targeting UBR5 inhibits postsurgical breast cancer lung metastases by inducing CDC73 and p53 mediated apoptosis.
    Article Snippet: .. To construct pLKO-Tet-shUbr5, pLKO-tet-scrambled and pLKO-TetshCdc73 plasmids, Ubr5-shRNA (sequences are listed in Table S1) and scrambled shRNA Cdc73-shRNA were cloned into the Tet-pLKO-puro (Addgene, plasmid # 21915) and Tet-pLKO-neo vector (Addgene, plasmid #21916). .. To construct the pLKO-shUBR5 and pLKOshCDC73 plasmid, UBR5-shRNA and CDC73-shRNA was cloned into the pLKO.1-puro (Addgene, plasmid #8453) and pLKO.1-neo vector (Addgene, plasmid #13425).

    Article Title: The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts
    Article Snippet: .. HeLa rescue cells were established by cloning the same shINTS11 sequence into Tet-pLKO-neo vector (Addgene) and single clones were selected with G418 (500 μg/ml). shRNA-resistant N-terminal Flag-tagged WT or E203Q mutant INTS11 cDNA ( ) were cloned into Cumate-pLenti-Cloning-2A-GFP vector (ABM Inc.), and transfected into a shINTS11-Tet-pLKO-neo single clone. ..



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    Biological pathways and genes targeted by MSI2. ( A ) Pathway analysis of MSI2-regulated genes for diseases and functions. The analysis was conducted with high-confidence genes showing differential regulation in genes upregulated in the polysome upon MSI2 knockdown. Plotted are the top 20 pathways and corresponding –log 10 ( P -value). ( B ) Western blot for candidate genes CDK6 and EIF3A upon stable <t>lentiviral</t> expression of either control (scramble) shRNA or MSI2 shRNA. Also included are western blots for MSI2 (showing knockdown of MSI2 with shRNA expression) and loading control (β-actin). ( C ) Genome coverage plots of CDK6 and EIF3A corresponding to 3′UTR from iCLIP for MSI2. ( D ) Quantification of EIF3A and CDK6 western blots (compared with loading control) from three replicates (β-actin). * denotes a P -value of 0.033 and ** denote a P -value of 0.008. EIF3A changed by 1.61-fold and CDK6 changed by 1.72-fold from all the replicate gels with β-actin as loading control.
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    Image Search Results


    Biological pathways and genes targeted by MSI2. ( A ) Pathway analysis of MSI2-regulated genes for diseases and functions. The analysis was conducted with high-confidence genes showing differential regulation in genes upregulated in the polysome upon MSI2 knockdown. Plotted are the top 20 pathways and corresponding –log 10 ( P -value). ( B ) Western blot for candidate genes CDK6 and EIF3A upon stable lentiviral expression of either control (scramble) shRNA or MSI2 shRNA. Also included are western blots for MSI2 (showing knockdown of MSI2 with shRNA expression) and loading control (β-actin). ( C ) Genome coverage plots of CDK6 and EIF3A corresponding to 3′UTR from iCLIP for MSI2. ( D ) Quantification of EIF3A and CDK6 western blots (compared with loading control) from three replicates (β-actin). * denotes a P -value of 0.033 and ** denote a P -value of 0.008. EIF3A changed by 1.61-fold and CDK6 changed by 1.72-fold from all the replicate gels with β-actin as loading control.

    Journal: NAR Cancer

    Article Title: Integrative genome-wide analysis reveals EIF3A as a key downstream regulator of translational repressor protein Musashi 2 (MSI2)

    doi: 10.1093/narcan/zcac015

    Figure Lengend Snippet: Biological pathways and genes targeted by MSI2. ( A ) Pathway analysis of MSI2-regulated genes for diseases and functions. The analysis was conducted with high-confidence genes showing differential regulation in genes upregulated in the polysome upon MSI2 knockdown. Plotted are the top 20 pathways and corresponding –log 10 ( P -value). ( B ) Western blot for candidate genes CDK6 and EIF3A upon stable lentiviral expression of either control (scramble) shRNA or MSI2 shRNA. Also included are western blots for MSI2 (showing knockdown of MSI2 with shRNA expression) and loading control (β-actin). ( C ) Genome coverage plots of CDK6 and EIF3A corresponding to 3′UTR from iCLIP for MSI2. ( D ) Quantification of EIF3A and CDK6 western blots (compared with loading control) from three replicates (β-actin). * denotes a P -value of 0.033 and ** denote a P -value of 0.008. EIF3A changed by 1.61-fold and CDK6 changed by 1.72-fold from all the replicate gels with β-actin as loading control.

    Article Snippet: Lentiviral vector pLKO-Tet-On (Addgene plasmid #21916) was used to generate inducible knockdown clones of MSI2 and EIF3A in the K562 cell line ( ) (details are provided in the Supplementary Methods).

    Techniques: Knockdown, Western Blot, Expressing, Control, shRNA